Еhe anabolic properties of a new composition of leuzea and cranberry meal extracts in a ratio of 7:50, containing ecdisten and ursolic acid, respectively, have been investigates on a model of isolated overload of the skeletal muscle of the rats leg by the method of excision. Material and methods. The experiment was conducted on Wistar stock rats of both sexes weighing 220–250 g. At the beginning of the experiment, all animals underwent musculus gastrocnemius tenotomy (m.gastrocnemius), and then the composition has been intragastrically administered for 14 days. At the end of the experiment, the rats were decapitated, blood was taken to determine biochemical parameters (creatine phosphokinase activity, glucose and lactate level) and muscles from both legs were separated to determine the difference between the mass of the musculus soleus (m. soleus) operated on and the mass m.soleus of a non-operated paw. Results and discussion. A dose-dependent anabolic effect was established for the composition of leuzea and cranberry meal extracts. A gender difference was found, which was confirmed by the obtained values of creatine phosphokinase activity and glucose/lactate ratio. Conclusions. The composition of leuzea and cranberry meal extracts exhibits a dose-dependent anabolic effect in males and increase physical endurance in female rats on the model of isolated overload of the skeletal muscle of the leg of rats with tenotomy operation.

This work shows the ability of apolipoprotein A-I to influence the activity of lysosomal glycosidases in the liver of mice in a model of BCG-induced tuberculous inflammation. The aim of the investigation was to study the activity of lysosomal glycosidases in the liver of mice using a model of BCG-induced tuberculous inflammation after intravenous administration of apolipoprotein A-I. Material and methods. The studies were performed on male CBA mice weighing 20-22 g. Disseminated tuberculous inflammation was modeled by a single intraperitoneal injection of 0.5 mg of BCG vaccine. The activity of lysosomal glycosidases was assessed spectrofluorimetrically by the content of hydrolysis products of the corresponding fluorogenic substrates. Results. Tuberculous inflammation led to a pronounced decrease in the activity of lysosomal glycosidases in the liver. Thus, β-galactosidase was decreased 2.3 times, β-glucosidase – 2.8 times, β-glucuronidase – 2.5 times compared with healthy animals. Intravenous injection of apolipoprotein A-I to animals against the background of BCG infection prevented a significant decrease in the enzymatic activity of glycosidases and these values practically did not differ from the control values. Conclusions. Enzymatic activity of lysosomal glycosidases in the group of mice with intravenous administration of apolipoprotein A-I against the background of BCG infection was 1.5–2 times higher than the corresponding indicators in the group of animals with BCG-infection without administration of apolipoprotein A-I, i.e. without treatment.

Aim of the study was to screen the antimicrobial activity of pyrimidine derivative 3-[2-(1-naphthyl)-2-oxoethyl]-6-bromoquinazoline-4(3H)-oh with laboratory cipher VMA–13–06 in relation to pathogenic and opportunistic flora. Material and methods. Antimicrobial activity of VMA–13–06 was established in vitro against strains of Staphylococcus aureus, Klebsiella pneumonia, Streptococcus pyogenes, Escherichia coli, Acinetobacter baumannii and Citrobacter freundii by the method of serial dilutions, by forming rows with different concentrations of the compound under study. Results. VMA–13–06 was found to exhibit high antibacterial activity against S. aureus, S. pyogenes, E. coli and K. pneumonia at concentrations of 128 and 64 μg/ml comparable to the activity of the comparison drug norfloxacin. At a concentration of 32 μg/ml, the studied derivative is highly active against S. aureus and S. pyogenes and shows average activity against E. coli and K. pneumonia. The compound VMA–13–06 in dilution from 16 to 4 μg/ml is moderately active against the above-mentioned microorganisms. At concentrations from 2 to 0.25 μg/ml, the pyrimidine derivative is inactive against S. aureus, S. pyogenes, E. coli and K. pneumonia, in all dilutions – against C. freundii and A. baumannii. Conclusions. The results of a screening study indicate a pronounced bactericidal effect of VMA–13–06 against S. aureus, S. pyogenes, E. coli и K. pneumonia comparable to the comparison drug norfloxacin.

The aim of the study was to test the hypothesis on the possible reason for the decrease in the activity of matrix metalloproteinases (MMPs) as a consequence of the high concentration of their tissue inhibitors (TIMPs) in the blood serum of patients with type 2 diabetes mellitus (T2DM). Material and methods. In the experimental part of the article, we used the blood serum of patients under observation in the clinic of the Federal Research Center of Fundamental and Translational Medicine. According to the content of glycated hemoglobin (HbA1С) in blood serum, the patients were divided into 3 groups: at the stage of compensation (6.0–6.5 % HbA1С), at the stage of subcompensation (6.6–7.0 % HbA 1С) and decompensation (> 7.0 % HbA1С). The activity of MMPs 2 and 7 in blood serum samples was measured by a fluorimetric method using a fluorescent substrate specific for these MMPs. The concentration of TIMP-1 (inhibitor of all non-membrane-bound MMPs) and TIMP-2 (active against MMP-2 and -7) in blood serum were determined by enzyme immunoassay. Results and discussion. In patients with T2DM, the MMP-2 and -7 activities decreased, more pronouncedly at the stage of decompensation. An increase in the concentration of TIMP-1 was observed in the serum of all patients, while no significant changes in the content of TIMP-2 were found. At the stage of decompensation, a decrease in MMP activity was accompanied by a decrease in the content of insulin, C-peptide and a corresponding increase in the level of proinsulin. An inverse correlation was found between the concentrations of TIMP-1 and insulin in patients at the stage of decompensation of T2DM. It is assumed that the activity of MMP-2 and -7, in comparison with their inhibitors, forms stronger correlations with the parameters of carbohydrate metabolism.

Our study aimed to explore associated non-alcoholic fatty liver disease (NAFLD) and metabolic liver dysfunction influence on the severity of the new coronavirus infection COVID-19. Material and methods. The study design was a cross-sectional study. The research included 215 patients (39.50 % of men) aged 26–60 years who had undergone a new coronavirus infection COVID-19 at least two months ago. Participants were divided into three groups by severity of infection: mild (n = 99), moderate and severe (n = 116) by anamnesis. Hepatic steatosis index (HIS), body mass index (BMI), waist circumference, alanine aminotransferase and gamma-glutamyl transpeptidase activity, glucose and triglyceride content, systolic and diastolic pressure were calculated and abdominal ultrasound examination was done. Results. In the group with moderate and severe course of COVID-19, the proportion of patients diagnosed with NAFLD
according to the HSI index was significantly higher compared to patients with mild coronavirus infection. Patients with mild COVID-19, who were diagnosed with NAFLD, had higher alanine aminotransferase and gamma-glutamyl transpeptidase activity, glucose and triglyceride content, BMI, systolic and diastolic pressure, waist circumference compared to patients without NAFLD. Similar differences persisted for patients with moderate and severe course. With the step-by-step exclusion of cardiometabolic parameters from the logistic regression model, the triglyceride content and BMI retained association with steatohepatosis according to ultrasound data, regardless of severity. When creating a similar model for the HSI index, significant correlation was shown for alanine aminotransferase activity in patients with mild COVID-19, for alanine aminotransferase activity and BMI – in patients with moderate and severe COVID-19. Conclusions. Patients with NAFLD have a more severe course of COVID-19. In addition, associations of the severity of COVID-19 with a combination of NAFLD and other cardiometabolic changes in the body, such as arterial hypertension, obesity, dyslipidemia, were revealed.

Acute appendicitis still predominates among surgical diseases. Despite the improvement in the technique of surgical intervention and treatment, the frequency of its complications ranges from 2 to 14%, which requires improvement of measures to prevent complications during operations and in the postoperative period. The aim of our work was to evaluate the effectiveness of the use of regional lymphatic stimulation and ozonized solutions in the prevention of complications after appendectomy with an atypical location of the appendix. Material and methods. The analysis included 45 patients with an atypical location of the appendix. Retrocecal was found in 25, retroperitoneal – in 12, and mesoceacal – in 8 patients. To assess the preventive measures used, 2 groups were identified. The comparison group consisted of 21 patients who received traditional treatment after surgery (to prevent complications antibiotics – ampicillin 1.0 g 4 times or gentamicin 80 mg 2 times a day for 4–5 days), pain relievers and infusion therapy. The main group included 24 patients who received regional lymphatic stimulation of the transitional fold of the peritoneum of the ileocecal region and the mesentery of the ileum, irrigation of the bed of the removed process and the surgical wound before suturing with ozonized sodium chloride solution with an ozone concentration of 8–10 μg/ml. In the diagnosis of appendicitis, clinical, laboratory (leukocyte count, leukocyte intoxication index) signs of the disease, and ultrasound were used. Results. In the comparison group, after surgery, suppuration appeared in 4 patients, wound infiltration was in 3 cases, early adhesive intestinal obstruction emerged once. Relaparatomy was performed with a favorable outcome. In the main group, one case of suppuration and infiltration of a postoperative wound was observed. By the time of discharge, all complications have been cured. There were no lethal outcomes. The hospital stay in the comparison group was 8.1 ± 0.37 bed-days, in the main group – 6.6 ± 0.12. Conclusions. The use of regional lymphatic stimulation can reduce the incidence of complications and shorten the length of hospital stay.

The morphology of the precentral gyrus is of particular interest because it is the origin of the pyramidal pathway responsible for voluntary movements. The aim of the study was to determine the width of the precentral gyrus, the thickness of the cerebral cortex in it and X-ray density of neurons in young boys and girls according to X-ray computed tomographic examination. Material and methods. 35 male and 37 female with mesocephalic sculls aged 18 to 20 years old with predominance of the right hand and no pathology of the nervous system organs in the anamnesis were examined. Results. The maximal value of the precentral gyrus width was found at the point located above the superior temporal gyrus. Values were lower (p < 0.01) at the point located at the level of the middle frontal gyrus. The smallest precentral gyrus width was found at a point above the cingulate gyrus (p < 0.01). When comparing the indices in each point, there is a tendency for them to be slightly more prevalent in the left hemisphere than in the right. There is a tendency to nonsignificant prevalence in boys compared to girls (p > 0.05). Parameters of cortical thickness in the precentral gyrus in the studied points did not differ statistically significantly, but there was a tendency to their insignificant predominance in the left hemisphere compared to the right, as well as in boys compared to girls (p > 0.05). Values of the X-ray neuron density in the compared points of the precentral gyrus in both hemispheres did not differ statistically significantly for boys and girls. Conclusions. The established results will serve as the initial data as a criterion of age-specific morphological norm in young mesocephals with predominance of the right hand, which is highly demanded in the personalized approach in medical practice. These results can also be used in prospective clinical and basic research.

Introduction. The COVID-19 pandemic has become a huge challenge for the entire healthcare system, medical institutions and doctors. The results of the industry’s activities turned out to be insufficiently effective, and therefore it is necessary to analyze not only what is directly related to the coronavirus infection, but also the consequences that the pandemic has led to. Goal. To analyze the results and consequences of health care activities and the health status of the population of the Siberian Federal District during the COVID-19 pandemic. Material and methods. The statistical data of the Ministry of Health of the Russian Federation, Rosstat, the Central Research Institute for the Organization and Informatization of Healthcare (TSNIIOIS), the Federal Service for Supervision of Consumer Rights Protection and Human Well-Being, as well as data for the Siberian Federal District were used. Scientific publications on the problem under consideration have been studied. Results and discussion. In connection with the beginning of the COVID-19 pandemic in Russia, as in all countries of the world, serious anti-epidemic measures were carried out, the procedure for providing medical care to the population changed, the consequences of which did not have the best effect on their health, including in the Siberian Federal District. The number of visits per resident of the district as a whole decreased by 16.2 %, in the polyclinic by 19.5 %, at home increased by 44.4 %. The number of visits for preventive purposes decreased by 6 %, and the coverage of dispensary observation by 11.2 %. The proportion of patients with oncological diseases in stages 3-4 increased to 40.6 %. Hospitalization of the SFO declined by 17.2 %, the number of operated patients 17.8 %, and postoperative complications have increased by 43.7 % from 1.07 to 1.54 %. Total mortality amounted to in 2020 for 14.5 per 1,000 population, i.e. increased by 18 % compared to 2019 year, while only 1/3 was due to COVID-19. In 2020, the number of Russians decreased by 510 thousand people. Conclusions. The results of health care activities, a significant deterioration in the health of the population and the complication of the demographic situation in the country during the pandemic, indicate serious shortcomings in the organization of medical care to the population, primarily in extreme situations, and requires a deep analysis and adequate measures to improve the structure and functioning of the industry in any conditions.

Acute, non-varicose bleeding from the upper gastrointestinal tract remains a common clinical problem. Bleeding episodes are associated with high mortality and a significant economic burden on the health care system. Despite the continuing improvement in endoscopic treatment, recurrent bleeding and associated mortality are still a pressing issue. In addition to the well-established modalities of endoscopic hemostasis: injection, thermal, mechanical, used both as mono therapy and as part of a combination therapy, the review discusses the use of novel types of endoscopic devices. The results of the use of over-the-scope clips, coagrasper, hemostatic sprays, endoscopic angiography, radiofrequency ablation, cryotherapy and endoscopic suturing device are described. The technical aspects of their application, the issues of efficacy and safety, the advantages and limitations of methods for achieving final endoscopic hemostasis are considered. The methods of initial assessment and treatment strategies for recurrent bleeding and unsuccessful endoscopic hemostasis were also analyzed. Material and methods. Literature search was carried out using the following electronic information resources: CyberLeninka, PubMed, Nature Pathology, MEDLINE, PLoS ONE. Results. Сombined endoscopic hemostasis is a standard therapy in the treatment of gastrointestinal tract cerebral infections with an efficiency of 95-98 %, new modalities of endoscopic hemostasis are able, in some cases, to achieve final endoscopic hemostasis both in primary endoscopic treatment and in recurrent bleeding.

The article is devoted to the actual gynecological problem of women of reproductive age-polycystic ovarian syndrome (PCOS) and the possibilities of its pharmacotherapy. Difficulties in choosing the treatment of the disease are the need to take into account all clinical manifestations, not limited to menstrual disorders and infertility. PCOS is usually accompanied by hirsutism, obesity, decreased glucose tolerance, and other manifestations in various organ systems associated with metabolic disorders. These manifestations significantly reduce the quality of life of patients and also require pharmacological correction. Currently, the first line of drugs in the treatment of PCOS remains combined oral contraceptives, which have been used by women for decades, but, unfortunately, have multiple adverse reactions. The latter is the reason for the search for other methods of pharmacotherapy that are highly effective and safe. Metformin is one these glucose-lowering drugs, which shows effectiveness in the treatment of PCOS. Drugs from such groups as glucagon-like peptide-1 receptor agonists are at the research stage, and the development of treatment tactics using glucose-lowering drugs that have antagonism to glucagon is continuing. Expanding the choice of pharmacotherapy, thanks to the search for new, more effective and safe drugs and combining it with other treatment methods, makes it possible to control PCOS taking into account the individual characteristics of patients.

Thrombotic complications contribute significantly in morbidity and mortality of patients with Ph-negative myeloproliferative neoplasms (MPN) including polycythemia vera, essential thrombocythemia, and primary myelofibrosis. Vascular endothelium is essential component of hemostatic system, and its functionality failure plays important role in prothrombotic states development. This review comprises analysis of available data on assessment of endothelium state characteristics in Ph-negative MPN and their detection with different methods. The search of literature sources was carried out using PubMed and eLibrary databases. The analysis of research results obtained with the use of different estimation techniques indicates that patients with Ph-negative MPN are characterized by endothelium activation, damage, and, consequently, dysfunction. Endothelium abnormalities associated with myeloproliferation have an effect on all endothelial functions fulfillment. Endothelial dysfunction represents important component of thrombosis pathogenesis in Ph-negative MPN.

Aim. To study the relation between the consumption of the main macronutrients and food groups with the risk of fatal outcomes from cardiovascular diseases (CVD) in people with newly diagnosed diabetes and those who are informed about type 2 diabetes mellitus (T2DM). Material and methods. This prospective analysis included 681 people (284 men and 397 women) with T2DM at baseline HAPIEE (Health, Alcohol and Psychosocial factors In Eastern Europe) 2003 (05) – December 31, 2018 and averaged 14.7 ± 0.7 years. There were 207 incident CV mortality during follow-up period (107 men, 100 women). The questionnaire on the presence of T2DM included information on the awareness of individuals about the presence of T2DM (“Did the doctor tell you that you have T2DM?”). The actual nutrition data included information on the consumption of 147 foods. The hazard ratio was estimated using Cox regression. Results and discussion. In men who aware of T2DM, the risk of developing a fatal CV event decreases with an increase in the consumption of “all-season” fruits (HR 0.413; 95 % confidence interval (CI) 0.234-0.786) and increases with an increase in the consumption of chicken eggs (HR 1.907 95 % CI 1.051-3.437), and alcohol (HR 1.031; 95 % CI 1.001- 1.061). In men who are unaware of T2DM, with an increase in nut consumption, the risk of developing fatal CV events decreases (HR 0.821; 95 % CI 0.679-0.992) and increases with increasing consumption of dairy products (HR 1.105; 95 % CI 1.000-1.221). In women aware of T2DM, consumption of white bread increases the risk (HR 1.489; 95 % CI 1.105-2.004), on the contrary, consumption of black bread reduces the risk of an undesirable outcome (HR 0.575; 95 % CI 0.424-0.818). Conclusions. In men who aware of T2DM, the most significant risk factors were the poor all-season fruits intake and excessive chicken eggs consumption. In women with type 2 diabetes, an increase in white bread consumption is of importance to increasing the risk of an undesirable CV outcome, and an increase in black bread consumption counts for reducing the risk.