INFLAMMATION MARKERS in CORNEA PENETRATING WOUND

Abstract

Research purpose was to study the presence and local levels of inflammation markers (cytokines) in the anterior chamber and blood in patients with penetrating corneal wound. Material and methods. Thirty patients aged 24-55 years (mean age 31.2 ± 0.34 years) with penetrating corneal wound for 1-2 days from the moment of trauma were examined. The control group consisted of 30 patients aged 38-55 years (mean age 43.9 ± 0.21 years) hospitalized for surgical treatment - phacoemulsification of cataract without concomitant pathology. Anterior chamber aqueous humor sampling was made in all patients: in the experimental group during the primary surgical debridement of cornea penetrating wound, in the control group - during the phacoemulsification of cataract. Peripheral blood sampling also was made just before the operation. IL-1β, IL-1Ra, IL-4, IL-6, IFN-α, TNF-α levels, measured by EIA, and the expression of the herpes viruses (cytomegalovirus and herpes simplex virus I and II), measured by PCR, were studied. Results and discussion. The study showed that all the investigated cytokines were in the blood and in the AC aqueous humor of examined patients. High concentration of IL-1Ra, IL-1β, TNF-a и IL-4 was in the experimental group, as compared to control group. Systemic innate immunity was activated in the experimental group and was significantly higher in the C-reactive protein index in patients of the experimental group as compared to control group. Conclusion. An acute period of eye injury is accompanied by a local and systemic increase in proinflammatory and anti-inflammatory cytokines, which indicates an imbalance in the regulation of immune inflammation, as the basis for the implementation of immuno-inflammatory complications.