Effect of Astragalus physodes extract on animal behavioral responses under «social» stress

An experimental study is devoted to the investigation of the effect of extract Astragalus physodes on the behavioral reactions of laboratory animals in conditions of «social» stress. Material and methods. The work was carried out toward white outbred male rats of 6–8 months of age. All animals were divided into groups (n = 10): 1 – intact rats, which were singly in the cell (control group); 2 – animals exposed to «social» stress (victims/aggressors); 3 – individuals exposed to «social» stress and received extract Astragalus physodes by intragastric gavage at a dose of 50 mg/kg/day for 14 days, starting from the 21 days of the experiment (victims/aggressors). «Social» stress was modeled by the formation of aggressive (aggressors) and submissive (victim) types of behavior in conditions of paired sensory contact. In studying the behavioral reactions of rats, psychopharmacological tests were used in the standard modification: «Elevated plus maze» and «Tail hanging». Results and discussion. In conditions of «social» stress, in the tests «Elevated plus maze» and «Tail hanging» among aggressors and victims, there is an increase in the level of anxiety and depression, which is manifested in a decrease in the number of rearing, the time spent on the open arms of the labyrinth. The results of the study indicate the ability of the herb extract Astragalus physodes under conditions of «social» stress to level the state of anxiety, which is manifested in an increase in the motor and approximate research activity of laboratory animals. Conclusions. Thus, the herb extract Astragalus physodes eliminates the increased anxiety generated under the influence of stressogenic effects, thereby exhibiting psychomodulatory properties, which actualizes further in-depth pharmacological studies of this extract with the aim of possibly creating medicinal preparations based on it.

1. Kolesnikova L.R. Stress-induced changes in the life of the body. Vestnik Smolenskoy gosudarstvennoy meditsinskoy akademii = Vestnik of the Smolensk State Medical Academy. 2018;17(4):30–36. [In Russian].

2. Kruk J., Aboul-Enein B.H., Bernstein J., Gronostaj M. Psychological stress and cellular aging in cancer: A meta-analysis. Oxid. Med. Cell. Longev. 2019; 2019: 1270397. doi: 10.1155/2019/1270397

3. Ridout K.K., Ridout S.J., Guille C., Mata D.A., Akil H., Sen S. Physician-training stress and accelerated cellular aging. Biol. Psychiatry. 2019;86(9):725– 730. doi: 10.1016/j.biopsych.2019.04.030

4. Sazonova M.A., Sinyov V.V., Ryzhkova A.I., Sazonova M.D., Kirichenko T.V., Khotina V.A., Khasanova Z.B., Doroschuk N.A., Karagodin V.P., Orekhov A.N., Sobenin I.A. Some molecular and cellular stress mechanisms associated with neurodegenerative diseases and atherosclerosis. Int. J. Mol. Sci. 2021;22(2):699. doi: 10.3390/ijms22020699

5. Morshed S.A., Davies T.F. Understanding thyroid cell stress. J. Clin. Endocrinol. Metab. 2020;105(3):e66–e69. doi: 10.1210/clinem/dgz193

6. Koolhaas J.M., Boer S.F., Buwalda B., Meerlo P. Social stress models in rodents: Towards enhanced validity. Neurobiol. stress. 2017;6:104–112. doi: 10.1016/j.ynstr.2016.09.003

7. Doeselaar L., Yang H., Bordes J., Brix L., Engelhardt C., Tang F., Schmidt M.V. Chronic social defeat stress in female mice leads to sex-specific behavioral and neuroendocrine effects. Stress. 2021;24(2):168– 180. doi: 10.1080/10253890.2020.1864319

8. Panossian A. Understanding adaptogenic activity: specificity of the pharmacological action of adaptogens and other phytochemicals. Ann. NY Acad. Sci. 2017;1401(1):49–64. doi: 10.1111/nyas.13399

9. Panossian A., Brendler T. The role of adaptogens in prophylaxis and treatment of viral respiratory infections. Pharmaceuticals (Basel). 2020;13(9):236. doi: 10.3390/ph13090236

10. Totty M.S., Warren N., Huddleston I., Ramanathan K.R, Ressler R.L., Oleksiak C.R., Maren S. Behavioral and brain mechanisms mediating conditioned flight behavior in rats. Sci. Rep. 2021;11(1):8215. doi: 10.1038/s41598-021-87559-3

11. Sergalieva M.U., Mazhitova M.V., Samotrueva M.A. Plants of the genus astragalus: prospects of application in pharmacy. Astrakhanskiy meditsinskiy zhurnal = Astrakhan Medical Journal. 2015;10(2):17–31. [In Russian].

12. Jalsrai A., Biswas A., Suslov N.I., Martin J.V. Neuropsychopharmacological profile of Astragalus membranaceous var. mongholicus. Journal of Traditional Chinese Medical Sciences. 2019;6(3):254–262. doi: 10.1016/j.jtcms.2019.08.002

13. Samotrueva M.A., Mazhitova M.V., Sergalieva M.U., Yasenyavskaya A.L. Phytochemical characteristics of Astragalus vulpinus Willd. herb and psychomodulating activity of its extract. Khimiko-farmatsevticheskiy zhurnal = Pharmaceutical Chemistry Journal. 2021;55(2):40–45. [In Russian]. doi: 10.30906/0023-1134-2021-55-2-40-45

14. Kudryavtseva N.N. The sensory contact model for the study of aggressive and submissive behaviors in male mice. Aggres. Behav. 1991;17(5):285–291.

15. Samotrueva M.A., Teplyy D.L., Tyurenkov I.N. Experimental models of behavior. Yestestvennyye nauki = Natural Sciences. 2009;(2):140–152. [In Russian].

16. Ross S.M. Resistance for strength: the role of phytomedicine adaptogens in stress management. Holist. Nurs. Pract. 2020;34(5):314–317. doi: 10.1097/ HNP.0000000000000408

17. Bratkov V.M., Shkondrov A.M., Zdraveva P.K., Krasteva I.N. Flavonoids from the genus astragalus: phytochemistry and biological activity. Pharmacogn. Rev. 2016;10(19):11–32. doi: 10.4103/0973-7847.176550

18. Ullah A., Munir S., Badshah S.L., Khan N., Ghani L., Poulson B.G., Emwas A.H., Jaremko M. Important flavonoids and their role as a therapeutic agent. Molecules. 2020;25(22):5243. doi: 10.3390/ molecules25225243

19. Rendeiro C., Rhodes J.S., Spencer J.P.E. The mechanisms of action of flavonoids in the brain: Direct versus indirect effects. Neurochem. Int. 2015;89:126– 139. doi: 10.1016/j.neuint.2015.08.002

20. Roohbakhsh A., Parhiz H., Soltani F., Rezaee R., Iranshahi M. Neuropharmacological properties and pharmacokinetics of the citrus flavonoids hesperidin and hesperetin – a mini-review. Life Sci. 2014;113:1–6. doi: 10.1016/j.lfs.2014.07.029