Immunogenetic characteristics of potential donors of hemapoietic stem cells recruited in the North Caucasus
https://doi.org/10.18699/SSMJ20210107
Abstract
Aim of the study was to investigate the immunogenetic characteristics of the potential donors of hematopoietic stem cells recruited in the North Caucasus and the distribution features of HLA alleles and multilocus haplotypes. Material and methods. Next Generation Sequencing technology was used to identify HLA-A, -B, -C, -DRB1 and -DQB1 alleles from 2663 unrelated bone marrow volunteers living in the Chechen Republic, Stavropol region, Republic of Dagestan. Mass parallel sequencing was performed using the MiSeq™ System («Illumina Inc.», USA). HLA allele and haplotype frequencies were estimated via maximum-likelihood analysis from genotypic data through an expectationmaximization (EM) algorithm for unknown gametic phase using Arlequin v. 3.5.2.2. Results and discussion. In studied population, 47 HLA-A, 77 HLA-B, 39 HLA-C, 54 HLA-DRB1, 22 HLA-DQB1 alleles were selected. Thirteenth alleles, HLA-A*02:01, HLA-A*01:01, HLA-B*13:02, HLA-B*51:01, HLA-C*06:02, HLA-C*04:01, HLA-C*07:02, HLADRB1*07:01, HLA-DRB1*13:01, HLA-DQB1*03:01, HLA-DQB1*02:02, HLA-DQB1*06:03, DQB1*03:02 exhibit frequencies over 10 %. The highest frequency extended haplotype HLA-A*02:01-B*13:02-C*06:02-DRB1*07:01DQB1*02:02, was observed frequencies of 4,5 %. Routine HLA typing allowed us to define 13 new HLA alleles.
About the Authors
M. A. Loginova
Kirov Research Institute of Hematology and Blood Transfusion of FMBA
Russian Federation
Russian Federation
Maria A. Loginova, candidate of biological sciences
610027, Kirov, Krasnoarmeyskaya str., 72
D. N. Smirnova
Kirov Research Institute of Hematology and Blood Transfusion of FMBA
Russian Federation
Russian Federation
Daria N. Smirnova
610027, Kirov, Krasnoarmeyskaya str., 72
S. S. Kutyavina
Kirov Research Institute of Hematology and Blood Transfusion of FMBA
Russian Federation
Russian Federation
Svetlana S. Kutyavina
610027, Kirov, Krasnoarmeyskaya str., 72
O. A. Makhova
Kirov Research Institute of Hematology and Blood Transfusion of FMBA
Russian Federation
Russian Federation
Olga A. Makhova
610027, Kirov, Krasnoarmeyskaya str., 72
K. P. Kashin
Kirov Research Institute of Hematology and Blood Transfusion of FMBA
Russian Federation
Russian Federation
Konstantin P. Kashin, candidate of medical sciences
610027, Kirov, Krasnoarmeyskaya str., 72
I. V. Paramonov
Kirov Research Institute of Hematology and Blood Transfusion of FMBA
Russian Federation
Russian Federation
Igor V. Paramonov, doctor of medical sciences
610027, Kirov, Krasnoarmeyskaya str., 72
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Review
For citations:
Loginova M.A., Smirnova D.N., Kutyavina S.S., Makhova O.A., Kashin K.P., Paramonov I.V. Immunogenetic characteristics of potential donors of hemapoietic stem cells recruited in the North Caucasus. Сибирский научный медицинский журнал. 2021;41(1):69-80. (In Russ.) https://doi.org/10.18699/SSMJ20210107
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