STUDYING THE POSSIBLE MUTAGENIC PROPERTIES OF NEW MEDICINE ON THE BASIS OF COMPLEX LITHIUM CITRATE, ALUMINUM OXIDE AND POLYMETHILSILOXANE
V. I. Konenkov,
M. A. Korolev,
A. A. Churin,
O. L. Voronova,
O. V. Neupokoeva,
L. N. Rachkovskaya,
A. V. Shurlygina,
M. V. Robinson,
A. A. Kotlyarova,
T. V. Popova,
E. E. Rachkovskiy,
P. G. Madonov,
A. Yu. Letyagin https://doi.org/10.15372/SSMJ20190507
Abstract
Aim of the study was to investigate the possible mutagenic properties of a new drug based on a lithium-containing substance – a complex of lithium citrate, polymethylsiloxane and aluminum oxide. Material and methods. Methods for testing mutagenicity using chromosomal aberrations in the bone marrow cells of CBA mice and somatic recombination in Drosophila melanogaster were used. Results. It was shown that a single intragastric administration of drug at a dose of 5000 mg/kg and a fivefold course of administration at a dose of 400 mg/kg to CBA mice did not increase the level of cytogenetic disorders in bone marrow cells. The study of the lithium complex drug in a somatic mosaicism test revealed that the preparation at a dose of 2000 mg/kg does not increase the frequency of mutations in Drosophila melanogaster. Conclusion. A single intragastric administration of the studied drug at a dose of 5000 mg/kg and its course administration (400 mg/kg × 5) do not increase the level of cytogenetic disorders in the bone marrow cells of CBA mice. In the somatic recombination (mosaicism) test system on D. melanogaster, no increase in the appearance of mutant setae and spots on the body and head was observed when using yellow and singed markers. The results of the study indicate that the studied drug does not have mutagenic properties.
About the Authors
V. I. Konenkov
Research Institute of Clinical and Experimental Lymphology – Branch of Federal Research Center Institute of Cytology and Genetics of SB RAS
Russian Federation
Russian Federation
doctor of medical sciences, professor, academician of the RAS
630060, Novosibirsk, Timakov str., 2
M. A. Korolev
Research Institute of Clinical and Experimental Lymphology – Branch of Federal Research Center Institute of Cytology and Genetics of SB RAS
Russian Federation
Russian Federation
candidate of medical sciences
630060, Novosibirsk, Timakov str., 2
A. A. Churin
Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Scientific Research Center
Russian Federation
Russian Federation
doctor of medical sciences
634028, Tomsk, Lenin av., 3
O. L. Voronova
Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Scientific Research Center
Russian Federation
Russian Federation
candidate of biological sciences
634028, Tomsk, Lenin av., 3
O. V. Neupokoeva
Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Scientific Research Center
Russian Federation
Russian Federation
candidate of biological sciences
634028, Tomsk, Lenin av., 3
L. N. Rachkovskaya
Research Institute of Clinical and Experimental Lymphology – Branch of Federal Research Center Institute of Cytology and Genetics of SB RAS
Russian Federation
Russian Federation
candidate of chemical sciences
630060, Novosibirsk, Timakov str., 2
A. V. Shurlygina
Research Institute of Clinical and Experimental Lymphology – Branch of Federal Research Center Institute of Cytology and Genetics of SB RAS
Russian Federation
Russian Federation
doctor of medical sciences, professor
630060, Novosibirsk, Timakov str., 2
M. V. Robinson
Research Institute of Clinical and Experimental Lymphology – Branch of Federal Research Center Institute of Cytology and Genetics of SB RAS
Russian Federation
Russian Federation
doctor of biological sciences
630060, Novosibirsk, Timakov str., 2
A. A. Kotlyarova
Research Institute of Clinical and Experimental Lymphology – Branch of Federal Research Center Institute of Cytology and Genetics of SB RAS
Russian Federation
Russian Federation
candidate of biological sciences
630060, Novosibirsk, Timakov str., 2
T. V. Popova
Research Institute of Clinical and Experimental Lymphology – Branch of Federal Research Center Institute of Cytology and Genetics of SB RAS
Russian Federation
630060, Novosibirsk, Timakov str., 2
Russian Federation
630060, Novosibirsk, Timakov str., 2
E. E. Rachkovskiy
Research Institute of Clinical and Experimental Lymphology – Branch of Federal Research Center Institute of Cytology and Genetics of SB RAS
Russian Federation
Russian Federation
candidate of chemical sciences
630060, Novosibirsk, Timakov str., 2
P. G. Madonov
Research Institute of Clinical and Experimental Lymphology – Branch of Federal Research Center Institute of Cytology and Genetics of SB RAS
Russian Federation
Russian Federation
doctor of medical sciences
630060, Novosibirsk, Timakov str., 2
A. Yu. Letyagin
Research Institute of Clinical and Experimental Lymphology – Branch of Federal Research Center Institute of Cytology and Genetics of SB RAS
Russian Federation
Russian Federation
doctor of medical sciences, professor
630060, Novosibirsk, Timakov str., 2
References
1. Borodin Yu.I., Rachkovskaya L.N., Darneva I.S., Novoselova T.I. Enterosorbent Noolite. For physical and psychological rehabilitation. Novosibirsk: Sova, 2006. 221 p. [In Russian].
2. Durnev A.D., Guskova T.A., Revazova Yu.A., Merkulov V.A., Verstakova O.L., Zhurkov V.S., Sycheva L.P., Zhanataev A.K., Yurchenko V.V. Methods for genetic risk assessment. In: Mironov A.N, editor. Guidelines for pre-clinical trials of drugs. Part one. Moscow: Grif i K, 2012. 94–114. [In Russian].
3. Medvedev N.N. Practical genetics. M.: Nauka, 1968. 296 р. [In Russian].
4. Rebrova O.Yu. Statistical analysis of medical data. Application software package STATISTICA. Moscow: Media-Sfera, 2002. 312 p. [In Russian].
5. European Convention for the Protection of Vertebrate Animals Used for Experimental and other Scientific Purposes (ETS 123). Strasbourg, 1986.
6. Girardi P., Brugnoli R., Manfredi G., Sani G. Lithium in bipolar disorder: Optimizing therapy using prolonged-release formulations. Drugs R D, 2016; 16 (4): 293–302
7. Malhi G.S., Tanious M., Das P., Berk M. The science and practice of lithium therapy. Aust. N. Z. J. Psychiatry. 2012; 46 (3): 192–211
8. Pastor N., Kaplan C., Domínguez I., Mateos S., Cortés F. Cytotoxicity and mitotic alterations induced by non-genotoxic lithium salts in CHO cells in vitro. Toxicol. In Vitro. 2009; 23 (3): 432–438
9. Rubtsov N.B., Borisov Yu.M. Sequence composition and evolution of mammalian B chromosomes. Genes. 2018; 9 (10): pii: E490.
Review
For citations:
Konenkov V.I., Korolev M.A., Churin A.A., Voronova O.L., Neupokoeva O.V., Rachkovskaya L.N., Shurlygina A.V., Robinson M.V., Kotlyarova A.A., Popova T.V., Rachkovskiy E.E., Madonov P.G., Letyagin A.Yu. STUDYING THE POSSIBLE MUTAGENIC PROPERTIES OF NEW MEDICINE ON THE BASIS OF COMPLEX LITHIUM CITRATE, ALUMINUM OXIDE AND POLYMETHILSILOXANE. Сибирский научный медицинский журнал. 2019;39(5):62-67. (In Russ.) https://doi.org/10.15372/SSMJ20190507
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