Idiopathic infertility in men of different ethnic groups: 8-hydroxy-2`-deoxyguanosine as a marker of oxidative stress

Oxidative stress is a proven factor in male infertility. Recently, there is increasing evidence that 8-hydroxy-2'- deoxyguanosine (8-OHdG) is considered an accurate and sensitive biomarker of oxidative DNA damage. Studies that revealed ethnospecificity of the course of lipoperoxidation processes suggest differences in oxidation of not only lipids but also DNA in men of different ethnogroups. Aim of the study was to evaluate free-radical lipid oxidation intensity and 8-OHdG level as a possible marker of oxidative stress in men of different ethnic groups in idiopathic infertility.

Material and methods. The study involved 672 men with idiopathic infertility, representatives of Russian (n = 225) and Buryat (n = 447) ethnic groups. Two control groups of practically healthy men of the corresponding ethnic groups with realized reproductive function were formed. Lipid and DNA free-radical oxidation product content was determined using spectrophotometric, fluorimetric and enzyme immunoassay methods.

Results. It is confirmed that oxidative DNA damage may be important in the etiology of male infertility. In men with idiopathic infertility of the Caucasian ethnic group, a significantly higher level of 8-OHdG in serum was found compared to fertile men, indicating the presence of changes at the cellular DNA level. There were no such changes in the Mongoloids, which indicates a difference in the intensity of metabolic processes among representatives of different ethnic groups.

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