ACTIVITY OF FREE RADICAL OXIDATION AND CYCLOOXYGENASE EXPRESSION IN RATS KIDNEYS UNDER EXPERIMENTAL THERAPY OF OXALATE NEPHROLITHIASIS WITH TETRAPEPTIDE Leu-Ile-Lys-Met
https://doi.org/10.15372/SSMJ20190410
Abstract
The aim of the study was to investigate the effect of Leu-Ile-Lys-Met tetrapeptide on the activity of free radical oxidation (FRO) and the level of cyclooxygenase-1 (COX-1) and COX-2 expression in rat kidney with experimental oxalate nephrolithiasis. Material and methods. The experiments were carried out on 30 male rats Wistar runoff, divided into 3 groups: the comparison group (10 intact rats), the control group (6 weeks of experimental oxalate nephrolithiasis simulation, n = 10), the experimental group (6 weeks of experimental simulation oxalate nephrolithiasis + oral administration of Leu-Ile-Lys-Met tetrapeptide at a dose of 12 mg/kg from the 3rd to the 6th week, n = 10). The oxalate nephrolithiasis was simulated according to conventional ethylene glycol model. COX-1 and COX-2 concentration in rat urine at baseline and after 6 weeks of experimental nephrolithiasis was determined by enzyme immunoassay using the prostaglandin-endoperoxide synthase 1 and 2 (PTGS 1, 2) kit from «Cloud-Clone Corp.». In rat kidney homogenate, the activity indicators of FRO processes were determined by common methods. Results and discussion. At the background of the Leu-Ile-Lys-Met tetrapeptide, free radical kidney damage was weakened, which was manifested in the complete absence of morphological signs of nephrolithiasis, decrease of thiobarbituric acid-reactive product concentration and overall prooxidant activity as compared to the control group; glutathione peroxidase activity, on the contrary, increased by 1.5 times.
About the Authors
A. Yu. Zharikov
Altai State Medical University of Minzdrav of Russia
Russian Federation
Russian Federation
doctor of biological sciences
6560038, Barnaul, Lenin av., 40
N. N. Yakushev
Altai State Medical University of Minzdrav of Russia
Russian Federation
Russian Federation
candidate of medical sciences
6560038, Barnaul, Lenin av., 40
G. V. Zharikova
Altai State Medical University of Minzdrav of Russia
Russian Federation
6560038, Barnaul, Lenin av., 40
Russian Federation
6560038, Barnaul, Lenin av., 40
O. N. Mazko
Altai State Medical University of Minzdrav of Russia
Russian Federation
Russian Federation
candidate of biological sciences
6560038, Barnaul, Lenin av., 40
O. G. Makarova
Altai State Medical University of Minzdrav of Russia
Russian Federation
Russian Federation
candidate of pharmacological sciences
6560038, Barnaul, Lenin av., 40
V. M. Bryukhanov
Altai State Medical University of Minzdrav of Russia
Russian Federation
Russian Federation
doctor of medical sciences
6560038, Barnaul, Lenin av., 40
Yu. V. Korenovskiy
Altai State Medical University of Minzdrav of Russia
Russian Federation
Russian Federation
candidate of medical sciences
6560038, Barnaul, Lenin av., 40
O. V. Azarova
Altai State Medical University of Minzdrav of Russia
Russian Federation
Russian Federation
doctor of biological sciences
6560038, Barnaul, Lenin av., 40
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Review
For citations:
Zharikov A.Yu., Yakushev N.N., Zharikova G.V., Mazko O.N., Makarova O.G., Bryukhanov V.M., Korenovskiy Yu.V., Azarova O.V. ACTIVITY OF FREE RADICAL OXIDATION AND CYCLOOXYGENASE EXPRESSION IN RATS KIDNEYS UNDER EXPERIMENTAL THERAPY OF OXALATE NEPHROLITHIASIS WITH TETRAPEPTIDE Leu-Ile-Lys-Met. Сибирский научный медицинский журнал. 2019;39(4):78-84. (In Russ.) https://doi.org/10.15372/SSMJ20190410
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