Association of prooxidant-antioxidant disorders with the development of morphofunctional disorders in rats with subtotal cerebral ischemia
https://doi.org/10.15372/SSMJ20200404
Abstract
The aim of the study was to investigate morphofunctional disorders of neurons in the cerebral cortex and changes in the prooxidant-antioxidant state in rats after subtotal cerebral ischemia. Material and methods. The experiments were performed on 20 white outbred male rats. Surgical interventions were carried out under intravenous thiopental anesthesia (40–50 mg/kg). Subtotal cerebral ischemia was simulated by both common carotid arteries ligation. Animals were decapitated after 60 minutes of ischemia. Morphological and functional changes in the neurons of the parietal cortex and hippocampal CA1 field, and intensity of free radical oxidative processes (content of thiobarbituric acid reactive substances and thiol-disulfide system components) in brain homogenates were studied. Results and discussion. Brain ischemia has been followed by oxidative stress in brain tissue. The cellular metabolism has been disturbed under the oxidative stress that leads to a water-electrolyte imbalance, manifested by deformation of neuron bodies, wrinkling, and swelling. The changes in parietal cortex are to a greater extent expressed, as the neurons are more sensitive to oxygen deficiency.
About the Authors
N. E. Maksimovich
Grodno State Medical University
Belarus
Nataliya E. Maksimovich, doctor of medical science, professor
Belarus
Nataliya E. Maksimovich, doctor of medical science, professor
E. I. Bon
Grodno State Medical University
Belarus
Elizaveta I. Bon, candidate of biological science
Belarus
Elizaveta I. Bon, candidate of biological science
E. I. Troyan
Grodno State Medical University
Belarus
Elina I. Troyan, candidate of biological science
Belarus
Elina I. Troyan, candidate of biological science
N. A. Valko
Grodno State Medical University
Belarus
Nikita A. Valko
Belarus
Nikita A. Valko
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Review
For citations:
Maksimovich N.E., Bon E.I., Troyan E.I., Valko N.A. Association of prooxidant-antioxidant disorders with the development of morphofunctional disorders in rats with subtotal cerebral ischemia. Сибирский научный медицинский журнал. 2020;40(4):28-34. (In Russ.) https://doi.org/10.15372/SSMJ20200404
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