The effect of xenon inhalation on the hemostasis system and liver function of laboratory animals during the development of acute respiratory distress syndrome

Xenon, being an inert gas, does not form chemical bonds with proteins and has a complex effect on the organs and systems of the body through biophysical processes. The hemostasis system and liver function were studied in view of the fact that all enzymes of the procoagulant pathway, being serine proteases, are synthesized mainly in the liver in the form of inactive proenzymes and circulate in the blood in this form. The aim of the study was to evaluate the impact of inhaled xenon-oxygen mixtures on hemostasis system and liver function in rats with acute respiratory distress syndrome (ARDS).
Material and methods. 75 female outbred rats were used in the experiment. Under general anesthesia, ARDS was modeled by intratracheal administration of a solution of hydrochloric acid with pepsin, adjusted to pH 1.2. Correction of the induced effects was performed by inhalation of a Xe/O2 mixture at a ratio of 70 % / 30 %. Hemostatic parameters (prothrombin time, activated partial thromboplastin time, fibrinogen, antithrombin III concentration), as well as biochemical parameters (activity of aspartate and alanine aminotransferases, lactate dehydrogenase, triglyceride, C-reactive protein content) were measured.
Results. The use of an inhalation mixture of Xe/O2 in the context of ARDS leads to an increase in coagulation hemostasis parameters (prothrombin time and activated partial thromboplastin time), normalization of antithrombin III content compared to the ARDS group, as well as to decrease of biochemical to control values.
Conclusions. The conducted experimental study showed a positive effect of Xe/O2 inhalations on the studied vital parameters of the body during the development of ARDS.

1. Kurbangaliev R.I., Avseitseva T.Yu., Potapkina Yu.M. Experience of using xenon in the treatment of neurological patients. Innovative medical technologies in the field of neurology and related medical specialties: coll. thes. rep. IV Intern. conf., Moscow, November 13, 2013. Moscow, 2013. P. 75–81. [In Russian]. Available at: http://kremlin-neurology.ru/library/Conference_neuro-2013_itog.pdf (date of request: 09.09.2025)

2. Gerasimova Yu.Yu., Ermakov M.A. Neuroprotective effects of subnarcotic and narcotic concentrations of medical xenon. Vestnik soveta molodykh uchenykh i spetsialistov Chelyabinskoy oblasti = Bulletin of the Council of Young Scientists and Specialists of the Chelyabinsk Region. 2017;3(3):21–24. [In Russian]

3. Marshall H., Stewart N.J., Chan H., Rao M., Norquay G., and Jim M. In vivo methods and applications of xenon-129 magnetic resonance. Prog. Nucl. Magn. Reson. Spectrosc. 2021;122:42–62. doi: 10.1016/j.pnmrs.2020.11.002

4. McGuigan S., Marie D.J., O’Bryan L.J., Flores F.J., Evered L., Silbert B. Scott D.A. The cellular mechanisms associated with the anesthetic and neuroprotective properties of xenon: a systematic review of the preclinical literature. Front. Neurosci. 2023;17:1225191. doi: 10.3389/fnins.2023.1225191

5. Shcherbak N.S., Galagudza M.M., Shlyakhto E.V. The role of hypoxia-induced factor-1 (hif-1) in the cytoprotective effect of ischemic and pharmacological postconditioning. Rossiyskiy kardiologicheskiy zhurnal = Russian Journal of Cardiology. 2014;11(115):70–75. [In Russian]. doi: 10.15829/1560-4071-2014-11-70-75

6. Myasnikova V.V., Sakhnov S.N., Romanov A.V. Cytoprotective effect of xenon. Sovremennye problemy nauki i obrazovaniya = Modern Problems of Science and Education. 2023;1:93. [In Russian]. doi: 10.17513/spno.32446

7. Molchanov N.V., Potievskaya V.I., Pulina N.N., Shebzukhova E.H. Treatment of patients with acute coronary syndrome with xenon inhalation. Doktor.Ru = Doctor.Ru 2012;(10):85–89. [In Russian].

8. Mammadov M.N., Rodionova Yu.V., Yavelov I.S., Smirnova M.I., Dudinskaya E.N., Potievskaya V.I. Coronavirus infection from the perspective of an interdisciplinary approach. Round table discussion. Kardiovaskulyarnaya terapiya i profilaktika = Cardiovascular Therapy and Prevention. 2021;20(3):73–86. [In Russian]. doi: 10.15829/1728-8800-2021-2849

9. Guo T, Fan Y, Chen M, Wu X., Zhang L., He T., Wang H., Wan J., Wang X., Lu Z. Cardiovascular implications of fatal outcomes of patients with coronavirus disease 2019 (COVID-19). JAMA Cardiol. 2020;5(7):811–818. doi: 10.1001/jamacardio.2020.1017

10. Nikolaev L.L., Petrova M.V., Bolikhova N.A., Dobrovolskaya N.Yu., Potapov A.V. Xenon as a component of adjuvant therapy in chemotherapy for breast cancer patients. Effektivnaya farmakoterapiya = Effective Pharmacotherapy. 2014:(57):6–9. [In Russian].

11. Fedorov S.S., Mogilnikov S.V., Fedorov S.Yu., Antonov A.A. Changes in systemic hemodynamics in patients with arterial hypertension under the influence of xenon-oxygen mixture. Vestnik novykh meditsinskikh tekhnologiy = Journal of New Medical Technologies. 2012;19(2): 266–269. [In Russian].

12. Gutsol L.O., Egorova I.E., Seminsky I.Zh., Guzovskaya E.V., Serebrennikova S.N., Dmitrieva L.A. Coagulation hemostasis: Modern view, pathology, diagnostics. Baikal Medical Journal. 2025;4(2): 32–52. doi: 10.57256/2949-0715-2025-4-2-32-52. [In Russian].

13. Merkulov G.A. Course of pathohistological technique. Leningrad: Meditsina, 1969. 422 p. [In Russian].

14. Fedorova E.P., Filonova M.V., Dubskaya T.Yu., Vetoshkina T.V., Sandrikina L.A., Fomina T.I. Neupokoeva O.V., Minakova M.Yu., Churin A.A. Study of disorders of the body’s life support systems in conditions of experimental lung damage. Vestnik SurGU. Meditsina = Bulletin of SurGU. Medicine. 2024;17(4):75–81. [In Russian]. doi: 10.35266/2949-3447-2024-4-10

15. Kobayashi H., Tachi A., Hagita S. Time course of histopathology of bleomycin-induced pulmonary fibrosis using an intratracheal sprayer in mice. Exp. Anim. 2024;1(73):41–49. doi: 10.1538/expanim.23-0048

16. Shpichko A.I., Grebenchikov O.A., Molchanov I.V., Shabanov A.K., Shpichko N.P., Kadantseva K.K. Cardioprotective properties of xenon. Neotlozhnaya meditsinskaya pomoshch’, zhurnal imeni N.V. Sklifosovskogo = Russian Sklifosovsky Journal of Emergency Medical Care. [In Russian]. 2020;9(2):264–272. doi: 10.23934/2223-9022-2020-9-2-264-272

17. Weber N.C., Kandler J., Schlack W., Grueber Y., Frädorf J., Preckel B. Intermitted pharmacologic pretreatment by xenon, isoflurane, nitrous oxide, and the opioid morphine prevents tumor necrosis factor alphainduced adhesion molecule expression in human umbilical vein endothelial cells. Anesthesiology. 2008;108(2):199–207. doi: 10.1097/01.anes.0000299441.32091.ed

18. Grebenchikov O.A., Shpichko A.I., Yevseyev A.K., Molchanov I.V., Shabanov A.K., Khusainov S.Z., et al. Neuroprotective properties of xenon according to experimental studies (a literature review). Neotlozhnaya meditsinskaya pomoshch’, zhurnal imeni N.V. Sklifosovskogo = Russian Sklifosovsky Journal of Emergency Medical Care. 2020;9(1):85–95. [In Russian]. doi: 10.23934/2223-9022-2020-9-1-85-95

19. Gileva O.G. Biochemical markers of liver damage with a fructose-induced diet in rats. Voprosy biologicheskoy, meditsinskoy i farmatsevticheskoy khimii = Problems of Biological, Medical and Pharmaceutical Chemistry. 2020;23(9):53−58. [In Russian]. doi: 10.29296/25877313-2020-09-08

20. Zudova A.I., Mukhlynina E.A., Gette I.F., Solomatina L.V. Changes in haematological and biochemical blood parameters of C57Bl/6 mice in acute paracetamol poisoning. Sibirskiy nauchnyy meditsinskiy zhurnal = Siberian Scientific Medical Journal. [In Russian]. 2025;45(1):86–93. doi: 10.18699/SSMJ20250109

21. Dolgov V.V. Clinical Laboratory diagnostics: National guidelines. Moscow: GEOTAR-Media, 2025. 672 p.

22. Smolyankin D.A., Timasheva G.V., Khusnutdinova N.Yu., Baygildin S.S., Karimov D.O., Repina E.F. Determination of alkaline phosphatase and lactate dehydrogenase activity in blood serum of white rats after cadmium chloride peros administration. Meditsina truda i ekologiya cheloveka = Occupational Medicine and Human Ecology. 2020;(3):101–107. [In Russian]. doi: 10.24411/2411-3794-2020-10313

23. Krauss R.M., Grunfeld C., Doerrler W.T., Feingold K.R. Tumor necrosis factor acutely increases plasma levels of very low-density lipoproteins of normal size and composition. Endocrinology. 1990;127:1016–1021. doi: 10.1210/endo-127-3-1016

24. Konyshko N.A., Volynets L.I., Konyshko G.S. Lipid metabolism indicators in patients with SARSCoV-2 infection. Infektsionnye bolezni: novosti, mneniya, obuchenie = Infectious Diseases: News, Opinions, Training. 2024;13(1):58–66. [In Russian]. doi: 10.33029/2305-3496-2024-13-1-58-66

25. Potievskaya V.I., Shvetskiy F.M., Kuznetsov S.V., Potapov S.V. The current understanding of how xenon acts in the human body. Doktor.Ru = Doctor.Ru. 2017;(6):55–59. [In Russian].