The level of apoptosis in the cells of the neocortex and hippocampus of white rats after mild traumatic brain injury with the introduction of glyproline

Glyprolines have a wide range of pharmacological effects, including antioxidant effects. This can be used to correct functional and morphological disorders of the brain after traumatic brain injury (TBI) by inhibiting secondary damage, the prevention of which is the main way to improve TBI outcomes. The aim of the study was to investigate the level of apoptosis in the cells of the proper parietal lobe (PPL) and the CA1 field of the hippocampus of mature male Wistar rats after mild TBI and a course of glyproline RPGP (Arg-Pro-Gly-Pro). Material and methods. Rat brain tissues (n = 38) were used in the experiment. Mild TBI was modeled by free fall of a load. After the injury, the animals received intraperitoneal RPGP peptide at a dose of 0.1 mg/kg, dissolved in 0.9% NaCl solution or NaCl solution. The level of apoptosis in brain tissues was assessed by the expression of p53 antigen using immunohistochemistry. Results. On the 22st day after TBI, the number of glial cells that entered into apoptosis in layer II of the proper parietal lobe (PPL) of the Wistar rat brain increases. In animals subjected to simulated TBI and receiving RPGP intraperitoneally at a dose of 0.1 mg/kg for 21 days, the level of apoptosis did not increase. Conclusion. It was found that after a course of RPGP administration against the background of TBI, the level of apoptosis in neuroglial cells decreases.

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