Effects of pegylated hyaloronidase on behavioural responses and cardiac electrical activity
https://doi.org/10.18699/SSMJ20240516
Abstract
The widespread use of testicular hyaluronidase preparations determines the particular interest in the development of oral dosage form. The drug under development, which is hyaluronidase pegylated using electron beam immobilization technology (PEG-GIAL), requires an assessment of its safety. Identification of possible neurotoxic action, as well as the influence on cardiac function seems to be very relevant. Aim of the study was to investigate the effect of PEG- GIAL on behavioural reactions and electrical activity of the heart of experimental animals. Material and methods. Eight hundred of white outbred rats (male and female) were used as experimental animals. The effect of PEG-GIAL on the central nervous system was determined using the «open field with holes» test, examining such characteristics as emotional reactions and orientation-research behaviour. The functional state of the cardiovascular system was assessed by electrocardiography data. Results. PEG-GIAL administration in all studied doses does not lead to changes in the functional activity of CNS, does not have a pronounced effect on the indices of orientation-research behaviour and emotional reactions in laboratory animals. No statistically significant changes in the cardiogram of rats were revealed, including no intersex differences. Conclusions. PEG-GIAL administration in all studied doses has no toxic effect on the electrical activity of the heart and has no pronounced effect on changes in the functional activity of the CNS and behavioural reactions.
About the Authors
A. M. Shvetsova
Research Institute of Clinical and Experimental Lymphology – Branch of the Federal Research Center Institute of Cytology and Genetics of SB RAS ; Research Institute of Pharmacology and Regenerative Medicine E.D. Goldberg of Tomsk National Research Medical Center of RAS
Russian Federation
Russian Federation
Alexandra M. Shvetsova
630060, Novosibirsk, Timakova st., 2
634028, Tomsk, Lenina ave., 3
M. A. Korolev
Research Institute of Clinical and Experimental Lymphology – Branch of the Federal Research Center Institute of Cytology and Genetics of SB RAS
Russian Federation
Russian Federation
Maksim A. Korolev, doctor of medical sciences
630060, Novosibirsk, Timakova st., 2
A. A. Churin
Research Institute of Pharmacology and Regenerative Medicine E.D. Goldberg of Tomsk National Research Medical Center of RAS
Russian Federation
Russian Federation
Aleksey A. Churin, doctor of medical sciences
634028, Tomsk, Lenina ave., 3
K. I. Ershov
Research Institute of Clinical and Experimental Lymphology – Branch of the Federal Research Center Institute of Cytology and Genetics of SB RAS ; Novosibirsk State Medical University of Minzdrav of Russia
Russian Federation
Russian Federation
Konstantin I. Ershov, candidate of biological sciences
630060, Novosibirsk, Timakova st., 2
630091, Novosibirsk, Krasny ave., 52
P. G. Madonov
Research Institute of Clinical and Experimental Lymphology – Branch of the Federal Research Center Institute of Cytology and Genetics of SB RAS ; Novosibirsk State Medical University of Minzdrav of Russia
Russian Federation
Russian Federation
Pavel G. Madonov, doctor of medical sciences
630060, Novosibirsk, Timakova st., 2
630091, Novosibirsk, Krasny ave., 52
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