RESTORATION OF CORNEA ENDOTHELIUM FUNCTION (REVIEW)
https://doi.org/10.15372/SSMJ20190304
Abstract
Review highlights modern findings on molecular mechanisms of dysfunction of human corneal endothelial cells causes decline of vision. When water enters the corneal stroma, it disorganizes the regular arrangement of the collagen fibrils, which reduces corneal transparency. Corneal endothelial cells are responsible for keeping the dehydration state of the stroma by pumping out fluid. However, this layer of cells can become deficient, for example following intra-corneal surgery or because of a pathology. Corneal transplantation is currently the only treatment in order to restore vision following endothelial dysfunctions. The authors survey methodological problems and prospects for correction of endothelial cells dysfunction. Human endothelial cells do not proliferate in vivo because these cells arrest in the G1 phase of the cell cycle. Modern research showed that corneal endothelium cells could proliferate in special conditions. An alternative approach is to use human stem cells as an endothelial cells source. New methods and improved technique of storage and preservation of corneal grafts, palliative methods of surgical treatment aimed at reducing the hydration of the surface layers of the cornea are considered. The review includes consideration of works for endothelial tissue engineering using cell culture technologies. Endothelial keratoplasty limited by the technical difficulty of the procedure, a shortage of available grafts, and the potential for graft failure or rejection. These limitations are driving researchers to develop new approaches, such as methods of organ culture
About the Authors
G. S. Baturina
The Federal Research Center Institute of Cytology and Genetics of SB RAS;
Novosibirsk State University
Russian Federation
Russian Federation
Baturina Galina Sergeevna – candidate of biological sciences, senior researcher of the sector of cell molecular physiology
630090, Novosibirsk, Academician Lavrentyev av., 10,
630090, Novosibirsk, Pirogova str., 2
L. E. Katkova
The Federal Research Center Institute of Cytology and Genetics of SB RAS
Russian Federation
Russian Federation
Katkova Lubov Evgenyevna – candidate of biological sciences, researcher of the sector of cell molecular physiology
630090, Novosibirsk, Academician Lavrentyev av., 10
E. I. Solenov
The Federal Research Center Institute of Cytology and Genetics of SB RAS;
Novosibirsk State University;
Russian Federation
Russian Federation
Solenov Evgeny Ivanovich – doctor of biological sciences, assistant professor, professor, chief researcher of the sector of cell molecular physiology
630090, Novosibirsk, Academician Lavrentyev av., 10,
630090, Novosibirsk, Pirogova str., 2,
630073, Novosibirsk, Karl Marks av., 20
I. A. Iskakov
S. Fyodorov Eye Microsurgery Federal State Institution of Minzdrav of Russia, Novosibirsk Branch
Russian Federation
Russian Federation
Iskakov Igor Alexeevich – doctor of medical sciences, head of surgery block
630071, Novosibirsk, Kolkhidskaya str., 10
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Review
For citations:
Baturina G.S., Katkova L.E., Solenov E.I., Iskakov I.A. RESTORATION OF CORNEA ENDOTHELIUM FUNCTION (REVIEW). Сибирский научный медицинский журнал. 2019;39(3):28-34. (In Russ.) https://doi.org/10.15372/SSMJ20190304
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