sibmedСибирский научный медицинский журналСибирский научный медицинский журнал2410-25122410-2520ИЦиГ СО РАН10.15372/SSMJ20200206sibmed-372Research ArticleМЕДИКО-БИОЛОГИЧЕСКИЕ НАУКИBIOMEDICINEБезопасность применения кондиционной среды, полученной при направленной остеогенной индукции мезенхимальных стволовых клеток костного мозга крысThe safety of the use of the conditioned medium obtained by directed osteogenic induction of rat bone marrow mesenchymal stem cellsПокровскаяЛ. А.PokrovskayaL. A.

Любовь Анатольевна Покровская

634050, г. Томск, просп. Ленина, 36

Lyubov’ A. Pokrovskaya

634050, Tomsk, Lenin av., 36

pokrovskayal@ect-center.comhttps://orcid.org/0000-0002-6178-5329ШерстобоевЕ. Ю.SherstoboevE. Yu.

Евгений Юрьевич Шерстобоев, д.м.н., проф.

634050, г. Томск, просп. Ленина, 36634028, г. Томск, просп. Ленина, 3 

Evgeniy Yu. Sherstoboev, doctor of medical sciences, professor

634050, Tomsk, Lenin av., 36634028, Tomsk, Lenin av., 3 

sherstoboev_eu@pharmso.ruНадеждинС. В.NadezhdinS. V.

Сергей Викторович Надеждин, к.б.н.

308015, г. Белгород, ул. Победы, 85

Sergey V. Nadezhdin, candidate of biological sciences

308015, Belgorod, Pobedy str., 85

sergey_nadezhdin@yahoo.comДанилецМ. Г.DaniletsM. G.

Марина Григорьевна Данилец, д.б.н.

634028, г. Томск, просп. Ленина, 3

Marina G. Danilets, doctor of biological sciences

634028, Tomsk, Lenin av., 3

danilets_mg@pharmso.ruТрофимоваЕ. С.TrofimovaE. S.

Евгения Сергеевна Трофимова, к.м.н.

634028, г. Томск, просп. Ленина, 3

Evgeniya S. Trofimova, candidate of medical sciences

634028, Tomsk, Lenin av., 3

eugenie76@mail.ruЛигачеваА. А.LigachevaA. A.

Анастасия Александровна Лигачева, к.б.н.

634028, г. Томск, просп. Ленина, 3

Anastasiya A. Ligacheva, candidate of biological sciences

634028, Tomsk, Lenin av., 3

vittelli@mail.ruЧуринА. А.ChurinA. A.

Алексей Александрович Чурин, д.м.н.

634028, г. Томск, просп. Ленина, 3

Aleksey A. Churin, doctor of medical sciences

634028, Tomsk, Lenin av., 3

churin_aa@pharmso.ruДубскаяТ. Ю.DubskayaT. Yu.

Татьяна Юрьевна Дубская, к.м.н.

634028, г. Томск, просп. Ленина, 3

Tat’yana Yu. Dubskaya, candidate of medical sciences

634028, Tomsk, Lenin av., 3

tatatox@mail.ruНациональный исследовательский Томский государственный университетРоссияNational Research Tomsk State UniversityRussian FederationНациональный исследовательский Томский государственный университет; НИИ фармакологии и регенеративной медицины имени Е.Д. Гольдберга, Томский национальный исследовательский медицинский центр РАНРоссияNational Research Tomsk State University; Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center of RASRussian FederationБелгородский государственный национальный исследовательский университетРоссияBelgorod National Research UniversityRussian FederationНИИ фармакологии и регенеративной медицины имени Е.Д. Гольдберга, Томский национальный исследовательский медицинский центр РАНРоссияGoldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center of RASRussian Federation2020020520204024755Copyright © Покровская Л.А., Шерстобоев Е.Ю., Надеждин С.В., Данилец М.Г., Трофимова Е.С., Лигачева А.А., Чурин А.А., Дубская Т.Ю., 20202020Покровская Л.А., Шерстобоев Е.Ю., Надеждин С.В., Данилец М.Г., Трофимова Е.С., Лигачева А.А., Чурин А.А., Дубская Т.Ю.Pokrovskaya L.A., Sherstoboev E.Y., Nadezhdin S.V., Danilets M.G., Trofimova E.S., Ligacheva A.A., Churin A.A., Dubskaya T.Y.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://sibmed.elpub.ru/jour/article/view/372

Цель исследования: изучить цитотоксичность in vitro и подострую токсичность in vivo кондиционной среды (КС), полученной при направленной остеогенной индукции мезенхимальных стволовых клеток (МСК) костного мозга крыс. Материал и методы. Кондиционную среду получали при культивировании МСК костного мозга крыс, индуцированных в остеогенном направлении. Результаты и их обсуждение. КС МСК костного мозга крыс, индуцированных в остеогенном направлении, не обладает существенным цитотоксическим действием на культуру клеток аденокарциномы Эрлиха и мононуклеары периферической крови здоровых доноров in vitro. Введение КС МСК не оказывало негативного влияния на состояние экспериментальных крыс SD, общую массу животных и ее прирост. Патоморфологическое исследование не выявило отклонений от общепринятой нормы, связанных с действием вводимого препарата. Заключение. Применение КС МСК костного мозга крыс, стимулированных в остеогенном направлении, как in vitro в отношении линии опухолевых клеток (аденокарцинома Эрлиха) и мононуклеаров периферической крови здоровых доноров, так и in vivo при введении экспериментальным животным является безопасным.

The purpose of the study was to evaluate in vitro cytotoxicity and in vivo sub-acute toxicity of conditioned medium obtained from rat bone marrow-derived mesenchymal stem cells directed to osteogenic differentiation. Material and methods. Conditioned medium was obtained by culturing rat bone marrow-derived MSCs induced under osteogenic condition. Results and discussion. Conditioned medium from rat bone marrow-derived MSCs was shown to have no significant cytotoxic effect on Ehrlich adenocarcinoma cell culture and in vitro expanded peripheral blood mononuclear cells from healthy donors. The use of MSC-CM did not have a significant effect on the state of experimental SD rats, the total body weight and growth rate of animals. A pathomorphologic study revealed no any abnormalities associated with MSC-CM injection. Conclusion. The use of conditioned medium from rat bone marrow-derived mesenchymal stem cells stimulated under osteogenic condition was found to be safe for both in vitro studies (with respect to Ehrlich adenocarcinoma cell line and peripheral blood mononuclear cells from healthy donors) and in vivo studies (injection of MSC-CM to animals).

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The authors declare that there are no conflicts of interest present.