sibmedСибирский научный медицинский журналСибирский научный медицинский журнал2410-25122410-2520ИЦиГ СО РАН10.18699/SSMJ20250403sibmed-2333Research ArticleОБЗОРЫREVIEWSСовременное состояние диагностики возбудителей вирусных геморрагических лихорадок методом изотермической амплификацииCurrent state of diagnostics of pathogens of viral hemorrhagic fevers using isothermal amplification methodhttps://orcid.org/0000-0001-5181-0415КривошеинаЕ. И.KrivosheinaE. I.

Кривошеина Екатерина Ильинична

630559, Новосибирская область, р/п Кольцово

Ekaterina I. Krivosheina

630559, Novosibirsk Region, Kol’tsovo

katr962@mail.ruhttps://orcid.org/0009-0003-2330-025XБеличенкоК. Р.BelichenkoK. R.

Беличенко Ксения Руслановна

630559, Новосибирская область, р/п Кольцово,

630090, г. Новосибирск, ул. Пирогова, 2

Ksenia R. Belichenko

630559, Novosibirsk Region, Kol’tsovo,

630090, Novosibirsk, Pirogova st., 2

ksenbelichen@gmail.comhttps://orcid.org/0000-0002-7857-6822КарташовМ. Ю.KartashovM. Yu.

Карташов Михаил Юрьевич, к.б.н.

630559, Новосибирская область, р/п Кольцово

Mikhail Yu. Kartashov, candidate of biological sciences

630559, Novosibirsk Region, Kol’tsovo

mikkartash@yandex.ruhttps://orcid.org/0000-0003-1521-897XЧубЕ. В.ChubE. V.

Чуб Елена Владимировна, к.б.н.

630559, Новосибирская область, р/п Кольцово

Elena V. Chub, candidate of biological sciences

630559, Novosibirsk Region, Kol’tsovo

chub_ev@vector.nscГосударственный научный центр вирусологии и биотехнологии «Вектор» РоспотребнадзораРоссияState Scientific Center of Virology and Biotechnology “Vector”Russian FederationГосударственный научный центр вирусологии и биотехнологии «Вектор» Роспотребнадзора; Новосибирский государственный университетРоссияState Scientific Center of Virology and Biotechnology “Vector”; Novosibirsk State UniversityRussian Federation2025050920254542938Copyright © Кривошеина Е.И., Беличенко К.Р., Карташов М.Ю., Чуб Е.В., 20252025Кривошеина Е.И., Беличенко К.Р., Карташов М.Ю., Чуб Е.В.Krivosheina E.I., Belichenko K.R., Kartashov M.Y., Chub E.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://sibmed.elpub.ru/jour/article/view/2333

Работа представляет собой обзор метода петлевой изотермической амплификации (loop-mediated isothermal amplification, LAMP) и его применения для диагностики геморрагических лихорадок. Ее цель состояла в краткой характеристике изотермической амплификации и систематизации имеющихся данных о диагностике данным методом геморрагических лихорадок, вызываемых возбудителями, которые относятся к первой группе патогенности. В обзоре описывается принцип работы LAMP, основанный на применении цепь-вытесняющей активности ДНК-полимеразы. Подробно рассматриваются преимущества метода, такие как высокая чувствительность и специфичность, простота проведения и экономическая эффективность. Также обсуждаются различные способы детекции результатов LAMP, включая турбидиметрический метод, использование флуоресцентных красителей и иммунохроматографический анализ. Отмечается, что LAMP имеет некоторые ограничения, такие как сложность подбора праймеров и возможность ложноотрицательных результатов при наличии мутаций в геноме. Однако в целом метод представляется перспективным для быстрого обнаружения вирусов в полевых условиях. Приводится информация о разработанных диагностических тест-системах на основе LAMP для выявления РНК особо опасных вирусов, относящихся к семействам Filoviridae и Arenaviridae. Отмечается, что, несмотря на перспективность метода, на данный момент ни одна из описанных тест-систем не зарегистрирована и не производится на коммерческой основе. Заключение подчеркивает необходимость разработки и внедрения новых методов диагностики опасных вирусных инфекций для контроля эпидемиологической ситуации и разработки стратегии противоэпидемических мероприятий. LAMP рассматривается как перспективный метод для создания быстрых и экономически эффективных тестов.

The work is an overview of the loop-mediated isothermal amplification (LAMP) method and its application to diagnostics of hemorrhagic fevers. Its objective was to briefly characterize isothermal amplification and systematize the available data on diagnostics by this method of hemorrhagic fevers caused by pathogens belonging to the first pathogenicity group. The review describes the principle of LAMP operation, which is based on the use of chain-displacing activity of DNA polymerase. The advantages of the method, such as high sensitivity and specificity, simplicity of implementation and cost-effectiveness, are considered in detail. Various methods for detecting LAMP results are also discussed, including the turbidimetric method, the use of fluorescent dyes and immunochromatographic analysis. It is noted that LAMP has some limitations, such as the complexity of primer selection and the possibility of false negative results in the presence of mutations in the genome. However, in general, the method seems promising for rapid detection of viruses in the field. The review provides information on the developed diagnostic test systems based on LAMP for detecting RNA of especially dangerous viruses belonging to the Filoviridae and Arenaviridae families. It is noted that despite the promise of the method, at present none of the described test systems are registered or produced on a commercial basis. The conclusion emphasizes the need to develop and implement new methods for diagnosing dangerous viral infections to control the epidemiological situation and develop a strategy for anti-epidemic measures. LAMP is considered a promising method for creating rapid and cost-effective tests.

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The authors declare that there are no conflicts of interest present.